Exploitation of Enzymes for the Production of Biofuels: Electrochemical Determination of Kinetic Parameters of LPMOs

Lytic polysaccharide monooxygenases (LPMOs) consist of a class enzymes that boost the release oxidised products from plant biomass, in an approach is more eco-friendly than traditional ones, employing harsh chemicals. Since LPMOs are redox enzymes, they could possibly be exploited by immobilisation on electrode surfaces. Such requires knowledge kinetic and thermodynamic information for interaction enzyme with surface. In this work, novel methodology applied determination such parameters LPMO filamentous fungus Thermothelomyces thermophila, MtLPMO9H.